C2087-47

Caspase 8 (FLICE, MASH, Mch5)

Size

£588.00

SKU:: C2087-47
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide derived from the C-terminal sequence of the p18 fragment of human caspase-8.
Clone:: 3A51(1C12)
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Extra Details:: Apoptosis induced by CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 (MACH/FLICE/Mch5), leading to the release of the active subunits p18 and p10 of caspase-8. Activated caspase-8 is able to cleave and activate downstream caspases such as caspase-1, caspase-3, caspase- 6 and caspase-7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.