C2087-33A
Caspase 8 (FLICE, MASH, Mch5)
Size
£601.00
- SKU:
- C2087-33A
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- aa360-385 of the human Caspase 8 protein.
- Clone:
- 4H46
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
- Extra Details:
- Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-2ug/ml. Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2ug/ml (lane 1) and 0.5ug/ml (lane 2). Detects 55kD caspase-8 in Jurkat cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice