C2087-32
Caspase 8 (FLICE, MASH, Mch5)
Size
£518.00
- SKU:
- C2087-32
- Purity:
- Ascities
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full-length recombinant human Caspase 8 protein.
- Clone:
- FLICE 4-1-20
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; inhibitors (activation by receptor cluster) and effetors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initaitor caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 (ECL). A band is seen at 55kD representing the pro form of Caspase 8 Optimal dilutions to be determined by the researcher. Positive control: Human kidney 293 cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice