C2087-29J-ML550

Caspase 7, NT (Caspase-7, CASP 7, CASP7, CASP-7, Apoptosis-related Cysteine Peptidase, Apoptosis Related Cysteine Protease, Apoptotic Protease MCH3, CMH 1, CMH-1, ICE-like Apoptotic Protease 3, ICE LAP3, ICE-LAP3, MCH3) (MaxLight 550)

Size

£953.00

SKU:: C2087-29J-ML550
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 16aa at the N-terminal of human Caspase-7. Epitope: within aa30-80
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Extra Details:: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Caspase-7, a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumor necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways. Applications: Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires heat-induced antigen retrieval using sodium citrate pH 6.0. Optimal dilutions to be determined by the researcher. Positive Control: Skeletal muscle tissue Storage and Stability: Storeproduct at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice