Caspase 3, Full-length (CPP-32, Apoptain, Yama, SCA-1)

Size

£793.00

SKU:: C2087-22
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human full-length Caspase 3 fusion protein containing a histidine-6 tag.
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5–2ug/ml (will detect the 32kDa precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells; previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20ug per lane for minigels. Optimal dilution determined by the researcher. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blotting Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.