Caspase 3, Full-length (CPP-32, Apoptain, Yama, SCA-1) (APC)

Size

£1,023.00

SKU:: C2087-22-APC
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human full-length Caspase 3 fusion protein containing a histidine-6 tag.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Applications: Suitable for use in Western Blotting. Recommended Dilution: Western Blotting: 0.5–2ug/ml (will detect the 32kDa precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells; previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20ug per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blotting Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.