C2086-66C
Caspase 1 (Interleukin-1 Beta Converting Enzyme, ICE)
Size
£800.00
- SKU:
- C2086-66C
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa224-242, CEPHKTSDSTFLVFMSHGIREG, of human caspase-1.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.5mM EDTA, 0.03% Proclin, 30% glycerol.
- Extra Details:
- Caspase 1/ICE (Interleukin-1b Converting Enzyme) is expressed as a proenzyme of 45kD in many tissues. Upon stimulus it gets activated by proteolytic cleavage. Active Caspase 1 is a tetramer of two subunits p10 and p20 in 2;2 proportion. Caspase 1 (cystein aspartic-specific protease 1) cleaves its substrates after an aspartate residue. It cleaves Interleukin b into its active form which further exerts its proinflammatory effects. Overexpression of caspase 1 can induce apoptosis in fibroblast which can be inhibited by overexpression of Crm A, a protein from pox virus. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-4ug/ml Immunocytochemistry: 10-20ug/ml Immunoprecipitation: 4-8ug/ml Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice