C2085-72H
Casein Kinase 2 beta (Protein Kinase CK2, PKCK2, CK2b, CK2 beta, CKIIb, CK2B1, CSNK2B1)
Size
£843.00
- SKU:
- C2085-72H
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Met1-His153 of human CK2β expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Casein kinase 2 (CK2) is a ubiquitous, tetrameric serine/threonine kinase comprised of two catalytic subunits (CK2a and/or CK2a‘) and two identical regulatory subunits (CK2b). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2b undergoes phosphorylation at Ser-2, Ser-3 and Ser-209. CK2b also plays dual roles in the regulation of CK2 acivity. The C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice