C2085-54D
Casein Kinase 1, delta
Size
£876.00
- SKU:
- C2085-54D
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant human CK1δ, Arg316-Arg415
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms (a, γ1, γ2, γ3, δ and ε) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1δ phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity. Preparation: Sheep antibodies were raised against purified, E. coli-derived recombinant human CK1δ (rhCK1δ; aa 316-415). Polyclonal antibody was affinity-purified on a column derivatized with the recombinant protein and further purified by isolating the IgG fraction. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice