C2069-55-BIOTIN
Catenin, gamma (Plakoglobin) (Biotin)
Size
£1,023.00
- SKU:
- C2069-55-BIOTIN
- Purity:
- Purified immunoglobulin
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human gamma-catenin
- Clone:
- 2Q239 (11E4)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function. a-catenin is a 102kD protein that is associated with the cadherin indirectly through its interaction with b-catenin and g-catenin. b-Catenin and its homolog plakoglobin associate directly with the cadherin. Thus, the cell-cell adherens junction is a structure composed of the transmembrane cadherin, which is associated directly with either b-catenin or plakoglobin, which in turn assoctiates directly with a-catenin. a- Catenin then mediates the interaction between the cadherin-catenin complex and the actin cytoskeleton. a-Catenin has been characterized as a tumor suppressor, suggesting that it may have a regulatory function in relation to its structural role. Applications: Suitable for use in Immunoblot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Histochoice fixative is effective. Immunocytochemistry: Effective for A431 and HT1080 cell lines. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice