C2069-54B
Catenin, beta, phosphorylated (Tyr142)
Size
£594.00
- SKU:
- C2069-54B
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Syynthetic peptide corresponding to amino acid residues around tyrosine 142 of human β-Catenin, conjugated to KLH.
- Format:
- Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
- Extra Details:
- b-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of b-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and b-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of b-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of b-Catenin. This phosphorylation regulates b-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of b-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion. Applications: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested. Recommended Dilution: Western Blot: 1:500. Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at RT. ELISA: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice