C2069-51
Catenin, beta, phosphorylated (Ser45)
Size
£639.00
- SKU:
- C2069-51
- Application:
- WB
- Purity:
- Purified by Protein A and immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser45 of human beta-catenin. Species sequence homology: mouse, rat, canine and porcine
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3beta may destabilize beta-catenin by phosphorylating it at serines 33, 37 and threonine 41 (4). Serine 45 in beta-catenin is phosphorylated by CK1. This likely serves as a priming site for subsequent phosphorylation by GSK-3 (5-7). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 (Mr 92kD) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice