C2069-39
Catenin, p120, CT
Size
£787.00
- SKU:
- C2069-39
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant fusion protein corresponding to the C-terminus of mouse p120 Catenin
- Clone:
- 15D2
- Format:
- Supplied as a liquid, 0.05% sodium azide.
- Extra Details:
- Originally characterized as a prominent substrate for Src (14) and receptor protein tyrosine kinases (eg, EGF, PDGF, CSF-1) (6), p120ctn is a novel catenin (10, 13) which binds directly to the juxta- membrane region of classical cadherins (eg, E-cadherin)(3 ,11,13,18). Emerging evidence suggests a prominent role in the regulation of cadherin mediated adhesion (1,4,12). Most cells express multiple isoforms of p120 that arise by alternative splicing (8, 9, 11), some of which can be distinguished using monoclonal antibodies (17). Fibroblasts express mostly type 1 isoforms containing an N-terminal extension of 101 amino acids. Type I isoforms may be important for cell motility and are selectively recognized. Epithelial cells preferentially express shorter isoforms lacking this extension. Heterogeneous or negative p120 staining has been reported in breast (5) and colon carcinomas (7, 16) and decreased p120 expression in bladder tumors correlated with a poor prognosis (15). Loss of p120 or E-cadherin never occurs in epithelial tissues under normal circumstances, and its expression is probably mandatory for normal tissue architecture. Like its cousin b-catenin, p120 may have dual roles in both cell-cell adhesion and in nuclear signaling. Applications: Suitable for use in Immunofluorescence (canine), ELISA (immunogen), Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:250-1:500 ELISA: 1:1000-1:10,000 Western Blot: 1:1000 Immunoprecipitation (Native): 5-10ul/IP reaction Optimal dilutions to be determined by the researcher. Positive Control: MDCK cells (canine), NIH 3T3 cells (mouse), Va-2 fibroblasts (human), HeLa cells (human), and rat-2 fibroblasts. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice