C1384-03
Cardiotin
Size
£659.00
- SKU:
- C1384-03
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 100kD cardiotin subunit.
- Clone:
- SR-3
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
- Extra Details:
- Cardiotin is a recently discovered, high molecular weight protein complex (300kD) located in the mitochondrial membrane. The cardiotin structure exists of subunits of 60kD and 100kD. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. R2G and SR-2, SR-3 and SR-4, monoclonal cardiotin antibodies can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested Recommended Dilutions: Immunohistochemistry: frozen Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice