C1350
CARD9 (Caspase Recruitment Domain)
Size
£655.00
- SKU:
- C1350
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa521-536, DRENTTGSDNTDTEGS, of human CARD9. Species sequence homology: mouse (88%) and rat (82%)
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of Bcl-10 (1). CARD9 associates with Bcl-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kB. CARD9 is an upstream activator of Bcl-10 and NF-kB signaling. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml detects a band at 59kD. Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: P3113-99: PC-3 Cell Lysate Human Prostate Adenocarcinoma MDA-MB-361 cell lysate Immunocytochemistry: K0001-05: K562 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice