C1300-17-BIOTIN

Carcinoembryonic Antigen (CEA, CD66e) (Biotin)

Size

£940.00

SKU:: C1300-17-BIOTIN
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CD66e
Clone:: C365D3 (NCRC23)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen/paraffin): 1:1000, requires protein digestion pre-treatment of paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.