C1299-87O

Carcinoembryonic Antigen (CEA)

Size

£956.00

SKU:: C1299-87O
Purity:: Purified by Ion Exchange chromatography (~90% by SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human natural tumor-derived purified CEA antigen
Clone:: 9B35
Format:: Supplied as a liquid in 10mM Tris, pH 7.5, 0.15M sodium chloride, 0.05% sodium azide. Also available without azide. See C1299-87S.
Extra Details:: The carcinoembryonic antigen (CEA) is a member of a large family of glycoproteins and a useful tumor marker for adenocarcinoma. In the past, clinical utility of CEA antibodies in immunopathology has been limited by cross-reactivity with CEA-related antigens, particularly Non-Specific Cross-Reacting Antigen (NCA), in normal colon mucosa and human granulocytes. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry Staining, Brightness, 80; Cytoplasmic staining, 3+; Membrane staining, 2+; Background, 2+; Staining of mucus 2+ Optimal dilutions to be determined by the researcher. Recommended Pair: Capture: C1299-87W Detection: C1299-87O Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.