C1211-05

Carboxypeptidase Y

Size

£914.00

SKU:: C1211-05
Purity:: Purified by delipidation, salt fractionation and ion exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Carboxypeptidase Y [Baker’s Yeast]
Format:: Supplied as a liquid from PBS, pH 7.2, 0.01% sodium azide
Extra Details:: Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. Other applications have not been tested. Recommended Dilution: ELISA: 1:10,000-1:42,000 Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Baker’s yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.