C1209-50

Carboxypeptidase H (Enkephalin Convertase, Carboxypeptidase E, CPE, CPH, Prohormone-processing Carboxypeptidase) (Not for Export EU)

Size

£812.00

SKU:: C1209-50
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminus of Carboxypeptidase H, conjugated to KLH.
Format:: Supplied as a liquid, 0.02% sodium azide
Extra Details:: CPH is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Carboxypeptidase H precursor migrates at ~53kD while active enzyme migrates at ~48kD. Immunocytochemistry (AtT-20 Cell line): 1:100-1:500 Immunoprecipitation: 1:250-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.