C1204-08
Carboxypeptidase A2
Size
£761.00
- SKU:
- C1204-08
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NS0-derived recombinant protein corresponding to Leu18-His417 from human CPA2.
- Clone:
- 8B5
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (aa 1 to 16), a pro region (aa 17 to 112), and a mature chain (aa 113 to 417). The amino acid sequence of human CPA2 is 99.5%, 90%, 87%, 86% and 70% identical to that of chimpanzee, canine, rat, mouse and chicken. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western blot: 1ug/ml Using recombinant Human Carboxypeptidase A2/CPA2 Immunoprecipitation: 25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice