C1150-01
Carboxyl Terminal Modulator Protein (CTMP)
Size
£754.00
- SKU:
- C1150-01
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 17aa peptide sequence near the C-terminus of human CTMP
- Format:
- Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
- Extra Details:
- Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase (PIK3). Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated on Thr308 in the catalytic domain and Ser473 in the C-terminal regulatory domain. Several protein partners for PKB have been identified. CTMP, a carboxyl terminal modulator protein, binds to PKB-a and negatively regulates PKB activity by inhibiting phosphorylation of these PKB residues. CTMP (mouse 248aa, human 240aa, chromosome 1q21, 22-26kD) predominantly expressed in skeletal muscle, testis, uterus, brain, and kidney, with lower levels in heart, liver, and lung. The presence of multiple transcripts suggested that CTMP undergoes alternative splicing. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice