C1146-04

Carbonyl Reductase 1 (CBR 1, CRN, CBR, hCBR1)

Size

£642.00

SKU:: C1146-04
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-HGQFVSEKRVEQW, from the C Terminus of human Carbonyl Reductase 1 (NP_001748.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 0.2-0.5ug/ml; ~30-35kD band seen in Human Liver and Kidney lysates [Calculated MW of 30.4kD according to NP_001748.1. Immunohistochemistry: 1-2ug/ml; In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.