C1105-80E
Carbonic Anhydrase IX (CA9)
Size
£672.00
- SKU:
- C1105-80E
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a C-terminal sequence within aa400-459 of the human carbonic anhydrase IX. Cellular Localization: Cell membranes
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.025% sodium azide.
- Extra Details:
- Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:1000 Western Blot: 1:2000 Flow Cytometry: 1:1000 Immunohistochemistry (Frozen, paraffin): 1:1000. Optimal dilutions to be determined by the researcher. Positive Control: Renal carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice