C1105-65A
Carbonic Anhydrase VI (Salivary Carbonic Anhydrase, Gustin)
Size
£787.00
- SKU:
- C1105-65A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa18-308 of human Carbonic Anhydrase VI expressed in NSO cells (NP_001206). Species Sequence Homology: Bovine (66%), mouse (50%).
- Clone:
- 9H79
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Carbonic Anhydrase VI (CA6), also known as gustin and salivary carbonic anhydrase, is a secreted zinc metalloenzyme that is a major protein component of parotid saliva and milk. It carries out a mucosal protective function by regulating pH balance in the upper alimentary and respiratory tracts. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Intracellular Flow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-5x10e5 OVCAR-3 cells in a total reaction volume ≤ 200ul. For intracellular staining, cells must first be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice