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C1105-65

Carbonic Anhydrase VI (Salivary Carbonic Anhydrase, Gustin)

Size

£832.00

SKU:
C1105-65
Purity:
Purified by Protein G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
NS0-derived recombinant human CA6 (aa18-308) Accession # NP_001206
Clone:
7H212
Format:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:
Carbonic anhydrase VI (CA6), also known as gustin and salivary carbonic anhydrase, is a secreted zinc metalloenzyme that is a major protein component of parotid saliva and milk. It carries out a mucosal protective function by regulating pH balance in the upper alimentary and respiratory tracts. Mature human CA6 shares 66% and 50% amino acid sequence identity with bovine and mouse CA6, respectively. Applications: Suitable for use in Western Blot, Immunopreciptation and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, in a colorimetric detection system, the detection limit for CA6 is ~25ng/lane under reducing and non-reducing conditions. Immunopreciptation: 25ug/ml from conditioned cell culture medium. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice