C1105-02

Carbonic Anhydrase I

Size

£780.00

SKU:: C1105-02
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: E. coli-derived recombinant human CA1 (aa2-261) Accession # NP_001729
Clone:: 7H139
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Carbonic Anhydrase I (CAI) is a cytosolic enzyme with the highest levels in erythrocytes. CA1 is a very early marker for erythroid differentiation. The amino acid sequence of human CA1 is 99%, 84%, 81%, and 78% identical to that of chimpanzee, canine, rat, and mouse. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.