C1103-04F

Carbohydrate Sulfotransferase (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-2, GST-3, HEC-GlcNAc6ST, HEC-GLCNAC-6-ST, L-selectin ligand sulfotransferase, LSST)

Size

£761.00

SKU:: C1103-04F
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to aa29-386 from human CHST4 expressed in CHO cells (Q8NCG5).
Clone:: 11D5
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Human CHST4 is an N-acetylglucosamine 6-O sulfotransferase. It transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. CHST4 is a type II transmembrane protein and is localized to the Golgi. It is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Human CHST4 shares 72% aa sequence identity with the mouse ortholog. Applications: Suitable for use in Direct ELISA, Immunoprecipitation and Neutralization. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.