C1103-02
Carbohydrate Sulfotransferase 2 (CHST2, Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, GlcNAc6ST-1, Gn6ST, GN6ST, GST2, GST-2, N-acetylglucosamine 6-O-sulfotransferase 1)
Size
£837.00
- SKU:
- C1103-02
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Human CHST2 aa76- 530.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also known as high endothelial cells Nacetylglucosamine 6Osulfotransferase (HECGlcNAc6ST) or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O glycans, but also has activity toward core 3 type of O glycan 5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice