NCAPH (Condensin Complex Subunit 2, Barren Homolog Protein 1, Chromosome-associated Protein H, hCAP-H, Non-SMC Condensin I Complex Subunit H, XCAP-H Homolog, BRRN, BRRN1, CAPH, KIAA0074)

Size

£642.00

SKU:: C1077-04C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-TEDLSDVLVRQGD, from human NCAPH, at C-terminal (NP_056156.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml, observed in lysates of cell line K562 on ~110kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.