Cannabinoid Receptor 1, CT (CB1)

Size

£780.00

SKU:: C1075-71J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa461-472 of human CB1 receptor MSVSTDTSAEAL. This sequence is identical in mouse, rat, canine and bovine.
Format:: Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: The CB1 receptor is a G-protein coupled receptor that binds the active component of cannabis, Δ9-tetrahydrocannabinol. This antibody has been raised against the C-terminal (amino acids 461-472) intracellular region of the human CB1 receptor and can be used for western blotting.1 2 Human and rat CB1 receptors exhibit 97.3% homology at the amino acid level over the complete protein, and 100% homology within the peptide sequence used to make this antibody.3 4 This peptide exhibits no homology with the CB2 receptor. Based on the amino acid sequence, the CB1 receptor has a molecular weight of approximately 52,800.4 The CB1 receptor and the splice variant CB1a are localized mainly in the brain, whereas the CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.3 4 5 6 Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:200 (4ug/ml) Immunohistochemistry (paraffin embedded sections): 1:50 (4ug/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice