C1036-78A
CaM Kinase II, beta
Size
£761.00
- SKU:
- C1036-78A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified rat brain CaM Kinase.
- Clone:
- 3G56 (CBb-1)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- CaM Kinase II (Ca2+/calmodulin-dependent protein kinase II) is a multifunctional Ca2+/calmodulin-dependent serine/threonine kinase, which is able to phosphorylate a variety of proteins in response to Ca2+ signals. This kinase participates in the regulation of numerous cellular functions including: 1) metabolism of carbohydrates, lipids, and amino acids, 2) neurotransmitter synthesis and release, 3) regulation of ion flux through ion channels, 4) Ca2+ homeostasis, 5) cytoskeletal function, and 6) Ca2+—regulated changes in gene expression. CaM Kinase II is broadly expressed and is highly concentrated in brain particularly in cortical structure and hippocampus. This kinase also plays an essential role in induction of long-term potentiation. Purified rat brain CaM Kinase has an apparent molecular weight of ~540kD and consists of isozymes composed of various ratios of 54kD (a), 58kD (b’), 60kD (b), 59kD (g) and 60kD (d) in a holoenzyme containing 10–12 subunits. The primary difference in the subunit isoforms lies in the variable region located at the carboxyl-end of the calmodulin binding domain. Ca2+/calmodulin- stimulated autophosphorylation of CaM Kinase II occurs on multiple residues. In particular, autophosphorylation on residue Thr286/Thr287 within the regulatory domain results in full activation of the enzyme, and in the generation of Ca2+/calmodulin- independent activity. Autophosphorylation has also been reported to increase the affinity of CaM Kinase for Ca2+/calmodulin. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice