Calsarcin-2 (Myozenin-1)

Size

£767.00

SKU:: C1036-04F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 17aa peptide from mouse/human Calsarcins-2, conjugated to KLH. Epitope Location: ~N-terminus. Species Sequence Homology: Rat - 100%; Bovine - 94%.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types. Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle. A novel family of striated muscle-specific calcineurin-interacting proteins called calsarcins or Myozenins has been identified that interact and colocalizes with the Z-disc protein alpha-actinin. Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse. Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle. Besides calcineurin and a-actinin, calsarcins interact with other Z-disc proteins g-filamin, telethonin and TCAP. Calsarcin-2/CALS-2 (variously termed Myozenin-1 and FATZ) ~34kD, human 299-aa, mouse 296-aa, ~30% identity with CALS-1) is a globular protein with central glycine-rich domain flanked by a-helical regions. CALS-2 is expressed transiently in heart during early embryogenesis and later becomes restricted to skeletal muscle with weaker signals in adult prostate, placenta and pancreas. In contrast to CALS-1, the expression of Calsarcin-2 is restricted to fast-twitch skeletal fiber (Gastrocnemius), which receives intermittent, high frequency stimulation and exhibit lower intracellular calcium concentration. Calsarcin-2 interacts with other Z-disc proteins g-Filamin, ACTN-2, ACTN-3 and TCAP and possibly modulates both g-filamin and a-actinin dimerization. Based on its binding partners, Calsarcin-2 has been suggested to play a central role in myofibrillogenesis and as a candidate for limb-girdle muscular dystrophy and other related neuromuscular disorders. Applications: Western Blot: 1-10ug/ml using ECL technique. ELISA Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice