Calsarcin 1 (Myozenin 2, Calcineurin Binding Protein)

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£754.00

SKU:: C1036-04A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17aa from the C-terminus of mouse Calsarcins-1 (KLH). Species Sequence Homology: Human and rat (83%)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse. Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle. Besides calcineurin and alpha-actinin, calsarcins interact with other Z-disc proteins gamma-filamin, telethonin and TCAP. Because calcineurin responds to sustained, low amplitude calcium signals, calsarcins may serve to localize calcineurin in the vicinity of unique intracellular pool, where it can interact with specific upstream activators or downstream substrates. Therefore, calsarcins may play an important role in modulating the function and substrate specificity of calcineurin in striated muscle cells. Calsarcin-1 (CALS-1), also known as Myozenin-2 or MYOZ2, is a ~32kD protein (human/mouse 264aa, ~88% identity). Amino acids 217-240 of calsarcin-1 are necessary for its interaction with a-actinin-2 in the Z-disc of sarcomeric muscle fibers. It is expressed, throughout the development-cycle, in all striated muscle tissues. However, CALS-1 expression is localized in slow-twitch fibers (soleus and plantaris), which depends on chronic motor neuron stimulation that results in sustained cellular calcium. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Western Blot: 1-10ug/ml using ECL. Optimal dilutions to be determined by the researcher. Control Peptide: C1036-04B: Calsarcin 1, Mouse (Myozenin 2) Control Peptide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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