Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

Size

£726.00

SKU:: C1036-03A
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to the C-terminus of human Calreticulin, fused to KLH.
Format:: Supplied as a liquid in PBS, 0.08% sodium azide.
Extra Details:: Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 Immunofluorescence: 1:50 Optimal dilutions to be determined by the researcher. Positive Control: MDCK cells; MCF-7 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.