C1036-01D-PE

Calretinin (Calbindin, CAB29, CAL2) (PE)

Size

£1,070.00

SKU:: C1036-01D-PE
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to rat calretinin.
Clone:: 6B8.2
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Extra Details:: Calretinin is a neuron specific calcium binding protein (31kD) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective. Western blot: 1:1,000–1:3,000 Immunohistochemistry on rat brain tissue: IFA: 1:1,000–1:5,000 PAP: 1:1,000–1:5,000 The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100–1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining. Note: Applications are based on unconjugated antibody.