C1035-61

Calprotectin (MRP-8-MRP-14 Complex, S100A8-S100A9 Complex)

Size

£1,132.00

SKU:: C1035-61
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human blood monocytes
Clone:: 6A488 (27E10)
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: The calcium binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50. ~22kD band observed under non-reducing conditions; antibody does not recognize the single proteins S100A8 and S100A9 Flow Cytometry: 1:50. Extracellular expression on monocytes. HL-60, platelets and lymphocytes may be used as negative control. Immunohistochemistry (Frozen sections): 1:50. Recommended: acetone fixation; 0.1% hdyrogen peroxide treatment to reduce endogenous peroxidase activity. Inflammatory tissue may be used as positive control; normal human skin, lung or colon tissues may be used as negative control. Immunohistochemistry (paraffin sections): 1:50. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice