Calnexin - ER Marker

Size

£607.00

SKU:: C1035-26G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal portion of the rat Calnexin protein. Epitope: aa550-591. Cellular Localization: Endoplasmic reticulum.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry/Immunofluorescence: 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100, using antigen retrieval with sodium citrate buffer (pH 6.0). Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells, HeLa whole cell lysate, Mouse bladder tissue. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice