C1035-25B3
CAMK2A (Calcium/Calmodulin-Dependent Protein Kinase II, CaMKII2A)
Size
£642.00
- SKU:
- C1035-25B3
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-PRTAQSEETRVWHR, from the internal region of human Camk2a. Species Sequence homology: rat and canine.
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMKIIA is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml. ~55-65kD bands observed in Human Brain (Cerebellum) and Mouse Brain lysates (MW 55.3kD and 54.1) Immunohistochemistry(Paraffin): 3-6ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice