C1035-05

Calmodulin (Phosphodiesterase 3'-5'-Cyclic Nucleotide Activator)

Size

£754.00

SKU:: C1035-05
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the 21 C-terminal amino acids (aa128-148) of bovine calmodulin, conjugated with tyroglobulin
Clone:: 0.T.172
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. Applications: Suitable for use in Western Blot, Immunoprecipation and RIA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml detects calmodulin (~17kD) in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-05. Proteins were visualized using a Gt xMo IgG (HRP) and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates calmodulin from A431 RIPA cell lysate. RIA: 10ug/ml detects calmodulin in the range of 200-300ng. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Included Positive Antigen Control: A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice