C0115-19F
Calcitonin Receptor (CRT, CTR, CT-R, CTR1, CALCR)
Size
£832.00
- SKU:
- C0115-19F
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HEK293 human embryonic kidney cell line transfected with human Calcitonin R, Ala25-Ala474.
- Clone:
- 9j1 (462802)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Calcitonin receptor (CALCR) is a glycosylated 70kD seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternate splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%-72% aa sequence identity with mouse and rat CACLR. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells. Validated using MCF-7 human breast cancer cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice