C0114-06D

Calcineurin alpha (CNA, CALNA, PPP3CA, Serine/Threonine-protein Phosphatase 2B Catalytic Subunit alpha Isoform, CAM-PRP Catalytic Subunit, Calmodulin-dependent Calcineurin A Subunit alpha Isoform)

Size

£512.00

SKU:: C0114-06D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bovine brain calcineurin.
Clone:: 10K119
Format:: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Extra Details:: Calcineurin is a major calmodulin binding protein in the brain. It is a holoenzyme composed of a calmodulin binding subunit, a catalytic alpha subunit (calcineurin A) and a calcium binding beta subunit (calcineurin B). The calcineurin A subunit has at least two isoforms in bovine brain; alpha 1 (61kD) and alpha 2 (59kD). Application: Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.