C0114-05A
Calcineurin A/PP2B Catalytic Unit (Protein Phosphatase 2B, A subunit, beta isoform, CnA beta)
Size
£732.00
- SKU:
- C0114-05A
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to rat Calcineurin A/PP2B catalytic unit near the C-terminus.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Calcineurin/PP2B Aβ, also known as Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, and encoded by the gene PPP3CB/CALNA2/CALNB/CNA2, is a calcium dependent, calmodulin stimulated catalytic subunit protein phosphatase critical in the function of Calcineurin an important phosphatase governing immune responses such as T cell activation and the T cell immune response. Calcineurin is a heterodimeric protein consisting of a catalytic subunit Calcineurin A, which contains an active site dinuclear metal center, and a tightly associated, myristoylated, Ca(2+)-binding subunit, Calcineurin B. Calcineurin/PP2B Aβ is the beta isoform of the catalytic active site of the subunit A of Calcineurin. Calcineurin also plays pivotal roles in signaling transduction cascades in a variety of other immune cells as well as non-immune cells like myocytes and neurons. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detected Calcineurin A/PP2B catalytic unit in 10ug of human brain tissue lysate. Immunoprecipitation: 5ug immunoprecipitated Calcineurin A/PP2B catalytic unit in 500ug of mouse brain tissue lysate. Optimal dilution to be determined by researcher Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice