C0113-14

Calbindin 1, Bovine (CABP28K, CALB1, Spot35 protein, Cholecalcin)

Size

£767.00

SKU:: C0113-14
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified bovine CABP28K (Calbindin 1, CALB1, Spot35 protein, Cholecalcin) protein
Clone:: 3H6
Format:: Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. D28K is ~28kD. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum) to confirm antibody specificity. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.