C0108-35G
N-Cadherin
Size
£754.00
- SKU:
- C0108-35G
- Purity:
- Purified by ammonium sulfate precipitation and anion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Neuronal tissue of chick embryos
- Clone:
- 3G48 (NCD-2)
- Format:
- Supplied as a lyophilized powder from PBS, 1% BSA. No preservatives added. Reconstitute with 50ul sterile ddH2O. Dilute further with PBS, 1% BSA, 0.09% sodium azide.
- Extra Details:
- The cadherin superfamily of proteins are a group of calcium mediated cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin. Applications: Suitable for use in Immunohistochemistry, Western Blot, Inhibition, Immunoprecipitation, Affinity Chromatography, Epitope Mapping and Adhesion Inhibition. Other applications not tested. Recommended Dilution: Immunohistochemistry: 10ug/ml Western Blot: 1-10ug/ml Inhibition of N-cadherin-dependent cell-cell contact: 100ug/ml for adhesion blocking Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice