Cadherin N (CDH2, Cadherin 2, type 1, N-cadherin (neuronal), Cadherin-2, Cadherin-2 precursor, CD325, CD325 antigen, CDHN, CDw325, CDw325 antigen, NCAD, N-cadherin, Neural-cadherin, Neural-cadherin precursor)

Size

£1,205.00

SKU:: C0108-35C
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa808-827 of human N-cadherin.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA. Reconstitute with 200ul sterile ddH2O. Dilute with 20mM TBS, pH 7.4, 1% BSA, 0.1% sodium azide, 10mM calcium chloride. (PBS may cause precipitation). In case of immunohistochemistry on paraffin or frozen sections, Ca2+ is not necessary.
Extra Details:: Cadherins are transmembrane glycoproteins involved in Ca++ dependent cell adhesion (1). The cadherin family, including (E)pithelial-Cadherin, (N)euronal-Cadherin, (P)lacental-Cadherin and (R)etinal-Cadherin, plays an important role in tissue morphogenesis and structure. N-cadherin (136kD) is expressed in nerve cells, developing skeletal muscle, embryonic and mature cardiac muscle cells, and pleural mesothelial cells. Studies have indicated that analysis of E-cadherin and N-cadherin expression may be useful in differentiating pleural mesotheliomas (N-cadherin positive) and lung adenocarcinomas (E-cadherin positive). Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen and paraffin): 5-10ug/ml Western Blot: 5-10ug/ml Optimal dilution to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice