C0108-33

Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Size

£971.00

SKU:: C0108-33
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human cdh1. MW of Antigen: ~55kD
Clone:: 3F40
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Cdh1 protein is a limiting, substrate specific activator of anaphase-promoting complex (APC)-dependent proteolysis and it is required for the degradation of the APC substrates, such as Clb2 and Ase1. Cdh1 plays important role in mitotic regulation. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein G Western Blot: 1-2ug/ml for 2hrs at RT Optimal dilutions to be determined by the researcher. Positive Control: Jurkat or LS174T cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.