C0108-32-ML650
Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1) (MaxLight 650)
Size
£1,023.00
- SKU:
- C0108-32-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- T-47D breast carcinoma line. Cellular Localization: Cell junctions
- Clone:
- 2Q663 (67A4)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Cadherin E is a 123kD molecule of the cadherin family. One of the epithelial cell adhesion molecules, Cadherin E, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso-or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, Cadherin E plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Cadnerins constitute a familly of transmembrane glycoproteins involved in Ca2+ dependent cell-cell interactions. The members of this family are diferentially expressed in various tissues. One of the best characterized cadherins in Cadherin E, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40kD transmembrane and cytoplasmic part. Cadherin E is a functional antibody which inhibits cell-cell adhesion. Antibodies against Cadherin E are suitable to characterize epithelial cells. Cadherin E is involved in adhesion of epithelial cells (mostly homotypic and homophilic) and is expressed on immature erythroid cells. In addition, it blocks the interaction of Cadherin E molecules and therefore is suitable to study Cadherin E-mediated signal transduction. Applications: Suitable for use in Flow Cytometry, Western Blot, Functional blocking of Cadherin E. Not recommended for use in Immunoprecipitation. Other applications not tested. Positive Control: MCF-7 membranes Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice