Cadherin 6 (K-Cadherin, Fetal Kidney, CDH6)

Size

£869.00

SKU:: C0107-05B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Thr22-Ala615 from human Cadherin 6, expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110aa long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P and R. Type II include cadherin-5, -6, -8, -11 and -12. During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithlium. Applications: Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using recombinant human Cadherin-6/KCAD Fc Chimera Immunocytochemistry: 5-15ug/ml detects Cadherin 6 on MG-63 human osteosarcoma cell line Flow Cytometry: 0.25ug/1x10e6 cells using MG-63 cell line Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice