Cadherin 5 (L-CAM, Epithelial Cell Adhesion Molecule, Vascular Endothelial, VE, CDH5, CD144)

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£754.00

SKU:: C0107-04
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal region of the human VE-cadherin. Species Sequence Homology: Porcine (75%)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Vascular endothelial cadherin belongs to the cadherin family of proteins, which are calcium-dependent, protease-sensitive molecules that mediate homotypic cell-cell adhesion. Cadherins are transmembrane glycoproteins, localized at adherens junctions in cardiac cells and at intermediate junctions in intestinal epithelia (1). While the extracellular domain mediates interactions with adjacent cells, the intracellular domain interacts with the cytoplasmic a-, b-, and g-catenin proteins, anchoring the cadherins to the actin cytoskeleton (1). Cadherin family members exhibit differential expression patterns and different cell-binding specificities; VE-cadherin is selectively expressed by the endothelial cells of most types of blood vessels (2), concentrated at interendothelial junctions (1). VE-cadherin is the target of signaling pathways and agents such as VEGF, histamine, and thrombin, that increase vascular permeability (3). Endothelial cells containing a null mutation in the VE-cadherin gene demonstrate major alterations in functional behavior and the inability to organize vascular-like structures, highlighting the importance of VE-cadherin in the maintenance of vascular integrity and normal organ function (3). In addition, VE-cadherin expression has been identified exclusively in highly aggressive uveal melanoma cell lines as opposed to their poorly aggressive counterparts, suggesting that VE-cadherin expression may be a marker for cells that have undergone a vasculogenic switch (4). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: PC-3 prostate adenocarcinoma cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice