C0107-03B
Cadherin, pan
Size
£895.00
- SKU:
- C0107-03B
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N terminal lysine residue (24aa) conjugated to glutaldegyde to KLH. Cellular Localization: Cell membrane
- Clone:
- 8J460 (CH-19)
- Format:
- Supplied as a liquid, 15mM sodium azide.
- Extra Details:
- Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+-dependent cell-cell adhesion(1,2,3). The N-terminal part of these molecules is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and alpha-actinin. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 Immunofluorescence: 1:500-1:1000 on cultured cells using methanol/acetone fixation Immunohistochemistry (Paraffin): 1:500-1:1000 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice